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J Virol Methods. 2005 Apr;125(1):95-8. Epub 2005 Jan 28.

Persistence of DNA in cell cultures may jeopardize the analysis of human herpesvirus 6 dynamics by means of real-time PCR.

Author information

1
Laboratoire de Virologie, UPRES EA2387, CERVI, Groupe Hospitalier Pitié-Salpêtrière, 75013 Paris, France.

Abstract

The use of real-time PCR has been described previously for analysing both the replication kinetics and antiviral susceptibility of human herpesvirus 6 in MT4 cells. It is now reported that viral DNA persists in infected cell culture long after the end of lytic virus replication. Consequently, high levels of DNA may correspond to an absence of infectivity and late readout occurring after the exponential phase of virus growth may lead to misinterpretation of the results of susceptibility assays. These limitations must be borne in mind when using real-time PCR.

PMID:
15737421
DOI:
10.1016/j.jviromet.2004.12.001
[Indexed for MEDLINE]

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