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Inflamm Res. 2005 Jan;54(1):31-6.

Increase in macrophage elastase (MMP-12) in lungs from patients with chronic obstructive pulmonary disease.

Author information

1
Laboratoire de Pharmacodynamie et de Pharmacologie Moléculaire, INSERM U620, Université de Rennes 1, 2, avenue du Professeur Léon Bernard, 35043, Rennes Cedex, France.

Abstract

OBJECTIVE AND DESIGN:

Chronic obstructive pulmonary disease (COPD) is characterized by an inflammatory process and airway remodeling involving matrix metalloproteinases (MMPs). Thus, we analyzed the expression and release of MMP-12 (macrophage metalloelastase) in bronchoalveolar lavage (BAL) and lung tissue from COPD patients and control subjects.

METHODS:

Immunocytochemistry and immunochemistry were performed to analyze the expression of MMP-12 in BAL cells and bronchial biopsies. Western blotting was used for the evaluation of MMP-12 in BAL fluids.

RESULTS:

The number of MMP-12 expressing macrophages together with the staining intensity was higher in BAL samples from COPD patients than in controls subjects. Similar results were noted in bronchial biopsies with higher MMP-12 expression in COPD subjects than in controls. Enhanced MMP-12 level was also observed in BAL fluids from patient with COPD in comparison to control subjects.

CONCLUSION:

This study demonstrated that COPD patients produce greater quantities of MMP-12 than controls, which may be a critical step in the pathogenesis of COPD and emphysema.

PMID:
15723202
DOI:
10.1007/s00011-004-1319-4
[Indexed for MEDLINE]

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