Format

Send to

Choose Destination
Curr Opin Struct Biol. 2005 Feb;15(1):107-15.

Novel modes of protein-RNA recognition in the RNAi pathway.

Author information

1
EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany.

Abstract

Gene silencing mediated by RNA interference (RNAi) depends on short interfering RNAs (siRNAs) and micro RNAs (miRNAs). These RNAs have unique features, namely a defined size of 19-21 base pairs, and characteristic two-nucleotide single-stranded 3' overhangs and 5' monophosphate groups. These molecular features of siRNAs and miRNAs are produced by RNase III enzymes, which are a hallmark of gene silencing induced by double-stranded RNA. Recent structural studies of components of the RNAi pathway, including PAZ, Piwi and RNase III domains, as well as full-length Argonaute and viral p19 proteins, have revealed distinct and novel modes of sequence-independent recognition of the characteristic features of siRNAs and miRNAs in the RNAi pathway.

PMID:
15718141
DOI:
10.1016/j.sbi.2005.01.010
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center