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Lett Appl Microbiol. 2005;40(3):195-200.

Isolation and identification of lactic acid bacteria from soil using an enrichment procedure.

Author information

1
Institute of Enology and Viticulture, Yamanashi University, Kitashin, Kofu, Yamanashi, Japan.

Abstract

AIMS:

To survey, and identify and classify the ecological distribution of lactic acid bacteria from soil in Japan and Taiwan.

METHODS AND RESULTS:

Acid-producing bacteria were isolated from 68 soil samples, collected from Japan and Taiwan, in the rhizospheres of fruit trees, from the floor of a henhouse and around a horse farm. All isolates were identified by physiological and genetic tests. Thirty-two of the 54 isolates were identified as lactic acid bacteria (LAB), 16 as spore-forming lactic acid bacteria, five as Clostridium and one as Bacillus. These lactic acid bacteria represent five genera: Lactobacillus, Lactococcus, Enterococcus, Leuconostoc and Weissella.

CONCLUSIONS:

A high rate of isolating lactic acid bacteria was obtained from soil.

SIGNIFICANCE AND IMPACT OF THE STUDY:

This study suggests that soil may be a common source for the isolation of lactic acid bacteria.

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