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Genes Dev. 2005 Feb 15;19(4):445-52.

Genome-wide RNAi screen reveals a specific sensitivity of IRES-containing RNA viruses to host translation inhibition.

Author information

1
Department of Genetics, Harvard Medical School and Howard Hughes Medical Instutite, Boston, Massachusetts 02115, USA. scherry@genetics.med.harvard.edu

Abstract

The widespread class of RNA viruses that utilize internal ribosome entry sites (IRESs) for translation include poliovirus and Hepatitis C virus. To identify host factors required for IRES-dependent translation and viral replication, we performed a genome-wide RNAi screen in Drosophila cells infected with Drosophila C virus (DCV). We identified 66 ribosomal proteins that, when depleted, specifically inhibit DCV growth, but not a non-IRES-containing RNA virus. Moreover, treatment of flies with a translation inhibitor is protective in vivo. Finally, this increased sensitivity to ribosome levels also holds true for poliovirus infection of human cells, demonstrating the generality of these findings.

PMID:
15713840
PMCID:
PMC548945
DOI:
10.1101/gad.1267905
[Indexed for MEDLINE]
Free PMC Article
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