A South American opossum (Monodelphis domestica) is a model animal for studies on the health effects of exposure to ultraviolet radiation (UVR). As part of a broad evaluation of immune function in this animal, we have tested in vitro mitogenic responses using whole blood cultures. Lymphocytes proliferated in the presence of phytohemagglutinin (PHA), concanavalin A (Con A), and pokeweed mitogen (PWM), but were unresponsive to bacterial lipopolysaccharide (LPS).