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Curr Microbiol. 2005 Feb;50(2):96-101. Epub 2005 Jan 20.

Effect of the microbial feed additive Saccharomyces cerevisiae CNCM I-1077 on protein and peptide degrading activities of rumen bacteria grown in vitro.

Author information

1
Lallemand Animal Nutrition, Sébastien Masséglia, 19 rue des Briquetiers, BP59, 31702 Blagnac cedex, France. fchauc@clermont.inra.fr

Abstract

We investigated the potential of the ruminant feed additive Saccharomyces cerevisiae CNCM I-1077 on protein and peptide degrading activities of the rumen bacterial species Prevotella albensis M384, Streptococcus bovis 20480, and Butyrivibrio fibrisolvens 3071 grown in vitro. Alive or heat-killed yeast cells were added to bacterial cultures in a complex casein-glucose medium. After incubation of the cultures at 39 degrees C under O(2)-free CO(2), peptidase activities were determined in the absence or in the presence of yeasts. Protease activities were detected after PAGE in gelatin-copolymerized gels. In co-incubations of bacteria and live S. cerevisiae I-1077, proteinase activities were reduced compared to the activities in the bacterial monocultures. Measurement of peptidase activities and microbial enumerations in the co-incubations suggested that live yeasts and bacteria interacted in a competitive way, leading to a decrease in peptidase activities. The mechanism responsible for such an effect could be mainly a competition for substrate utilization, but the release of small competitive peptides by the yeast cells is also likely to be implicated.

PMID:
15702254
DOI:
10.1007/s00284-004-4433-1
[Indexed for MEDLINE]

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