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Biomaterials. 2005 Jul;26(21):4351-7.

Lysozyme and bovine serum albumin adsorption on uncoated silica and AlOOH-coated silica particles: the influence of positively and negatively charged oxide surface coatings.

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Nonmetallic Inorganic Materials, Department Materials, ETH Zurich, Switzerland.


The adsorption of lysozyme and bovine serum albumin on silica and AlOOH-coated silica particles-representing negatively and positively charged oxide surfaces-was investigated. The protein-treated uncoated and completely AlOOH-coated silica particles were characterized by zeta potential analysis and UV/VIS spectroscopy. It was found that at pH 7 a protein oppositely charged to the oxide surface adsorbs in significantly higher amounts. In contrast, proteins of the same charge did not or only in very low amounts adsorbed on an oxide surface. As both oxide surfaces were measured to be very hydrophilic it can be concluded that electrostatic interactions dominate the adsorption process at the investigated experimental conditions. The pH regions where the proteins interact via attractive and repulsive coulomb interaction with the particular oxide surfaces were calculated and outlined.

[Indexed for MEDLINE]

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