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Plant Cell Rep. 2005 May;24(2):127-32. Epub 2005 Feb 8.

Enhanced production of recombinant proteins from plant cells by the application of osmotic stress and protein stabilization.

Author information

  • 1Department of Chemical Engineering, Washington State University, Pullman, WA 99164-2710, USA. rsoder@wsu.edu

Abstract

Human granulocyte-macrophage colony-stimulating factor (GM-CSF) was produced from transgenic Nicotiana tabacum cells. The application of osmotic stress through the addition of 90 g/l mannitol to the plant cell medium enhanced the maximum extracellular GM-CSF concentration from 76 microg/l to 130 microg/l (1.7-fold increase). The addition of bovine serum albumin (BSA), along with mannitol, further increased the maximum extracellular GM-CSF concentration by as much as 2.5-fold over the control. GM-CSF degradation studies in conditioned medium demonstrated that mannitol and BSA both stabilize the GM-CSF protein. The addition of gelatin together with mannitol to the plant cell medium also enhanced the maximum extracellular GM-CSF concentration and stability over time.

PMID:
15700142
DOI:
10.1007/s00299-005-0918-z
[PubMed - indexed for MEDLINE]
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