Format

Send to

Choose Destination
Genome Biol. 2005;6(2):R19. Epub 2005 Jan 17.

A universal method for automated gene mapping.

Author information

1
Institute of Molecular Biology, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland. peder.zipperlen@molbio.unizh.ch

Abstract

Small insertions or deletions (InDels) constitute a ubiquituous class of sequence polymorphisms found in eukaryotic genomes. Here, we present an automated high-throughput genotyping method that relies on the detection of fragment-length polymorphisms (FLPs) caused by InDels. The protocol utilizes standard sequencers and genotyping software. We have established genome-wide FLP maps for both Caenorhabditis elegans and Drosophila melanogaster that facilitate genetic mapping with a minimum of manual input and at comparatively low cost.

PMID:
15693948
PMCID:
PMC551539
DOI:
10.1186/gb-2005-6-2-r19
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center