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Cell Transplant. 2004;13(7-8):823-31.

Maintenance of functional human cancellous bone and human hematopoiesis in NOD/SCID mice.

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1
Department of Cytology and Histology, University of Liège, Liège, Belgium. F.Hubin@ulg.ac.be

Abstract

Attempts were made to establish models to study interactions between marrow stromal cells and hematopoietic cells in vivo. The approach was to create a NOD-SCID-hu murine model of long-term human hematopoiesis by implantation of a human adult bone fragment. Nine to 12 weeks posttransplantation, human CD45+ cells were detected in the blood and the spleen of some mice. The histology of the human transplant showed that human bone fragment was viable at 9 weeks. Moreover, vessels of human origin, as assessed by immunohistochemical detection of human beta2-microglobulin, were observed in the mouse tissue surrounding the transplanted human fragment.

PMID:
15690985
DOI:
10.3727/000000004783983387
[Indexed for MEDLINE]
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