Format

Send to

Choose Destination
FEMS Microbiol Lett. 2005 Feb 15;243(2):405-9.

Assessment of a new technique combining a viability test, whole-cell hybridization and laser-scanning cytometry for the direct counting of viable Enterobacteriaceae cells in drinking water.

Author information

1
NSERC Industrial Chair on Drinking Water, Civil, Geological and Mining Engineering, Ecole Polytechnique de Montréal, Montréal, Qué., Canada H3C 3A7. baudart@obs-banyuls

Abstract

A new direct approach, called direct viable count (DVC)-FISH-ScanRDI, combining viability measurement, specific detection and sensitive enumeration of highly diluted Enterobacteriaceae cells, was assessed during the summer in water samples from a North American drinking water treatment plant and its distribution system. Major results of this field investigation show a higher sensitivity of the DVC-FISH-ScanRDI approach in enumerating viable Enterobacteriaceae cells in distributed drinking water, relative to a culture-based method, and the increased concentration of viable but non-culturable (VBNC) Enterobacteriaceae cells in distributed water for temperatures above 18 degrees C.

PMID:
15686842
DOI:
10.1016/j.femsle.2005.01.001
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for Wiley
Loading ...
Support Center