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J Antimicrob Chemother. 2005 Mar;55(3):383-6. Epub 2005 Jan 28.

Intracellular persistence of Escherichia coli in urinary bladders from mecillinam-treated mice.

Author information

1
Department of Bacteriology, Mycology and Parasitology, National Center of Antimicrobials and Infection Control and Department of Virology, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark. mbp@ssi.dk

Abstract

OBJECTIVES:

It has been suggested recently that intracellular bacteria surviving antibiotic treatment might serve as a reservoir for recurrent infection. The purpose of this study was to directly examine the location of Escherichia coli bacteria in the mouse bladder after treatment with mecillinam.

METHODS:

The bladders were studied by use of colony counts, in situ hybridization and electron microscopy.

RESULTS:

The bacterial counts in the bladder remained approximately 10(3-4) cfu/bladder even after mecillinam treatment had finished, and re-growth in the urine was observed. In the bladder epithelium from treated mice, bacteria cells were occasionally seen, presumably representing intracellularly located bacteria.

CONCLUSIONS:

This is the first in vivo study indicating that during mecillinam treatment E. coli cells can penetrate the mouse bladder epithelium and persist.

PMID:
15681580
DOI:
10.1093/jac/dki002
[Indexed for MEDLINE]

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