Abstract
The ethyl acetate soluble fraction of hops (Humulus lupulus) showed potent inhibitory activity on the production of nitric oxide (NO) induced by a combination of LPS and IFN-gamma. Four known prenylflavonoids (1-4) and a new prenylflavonoid (5), hulupinic acid (6), lupulone (7), and its six new derivatives (8-13) were isolated from the active fraction. The structures were determined on the basis of physiochemical properties and spectroscopic analysis. Their inhibitory activities on the production of NO in macrophage RAW 264.7 cells were examined.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cells, Cultured
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Enzyme Inhibitors / chemistry
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Enzyme Inhibitors / isolation & purification
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Enzyme Inhibitors / pharmacology*
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Flavonoids / chemistry
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Flavonoids / isolation & purification
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Flavonoids / pharmacology*
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Humulus / chemistry*
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Interferon-gamma / pharmacology
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects
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Macrophages / metabolism*
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Mice
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Nitric Oxide / antagonists & inhibitors*
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Nitric Oxide / biosynthesis
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Nitric Oxide Synthase / antagonists & inhibitors*
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Nitric Oxide Synthase / biosynthesis
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Nitric Oxide Synthase Type II
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Nuclear Magnetic Resonance, Biomolecular
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Phloroglucinol / analogs & derivatives*
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Phloroglucinol / chemistry
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Phloroglucinol / isolation & purification
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Phloroglucinol / pharmacology*
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Plants, Medicinal / chemistry*
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Terpenes / chemistry
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Terpenes / isolation & purification
Substances
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Enzyme Inhibitors
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Flavonoids
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Lipopolysaccharides
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Terpenes
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Nitric Oxide
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Interferon-gamma
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lupulon
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Phloroglucinol
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Nitric Oxide Synthase
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Nitric Oxide Synthase Type II
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Nos2 protein, mouse