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Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1329-32. Epub 2005 Jan 26.

Chromatin remodeling by DNA bending, not twisting.

Author information

1
Department of Structural Biology, School of Medicine, Stanford University, Stanford, CA 94305, USA.

Abstract

Single-stranded regions (gaps) in nucleosomal DNA interfere with action of the RSC chromatin-remodeling complex, monitored by exposure of restriction endonuclease cutting sites. Single-strand breaks (nicks) in the DNA, by contrast, have no effect. Gaps on one side of the cutting site are inhibitory, but gaps on the other side are not. A gap >100 bp from the cutting site is as effective as a gap <20 bp from the site. These findings suggest a remodeling process involving bending, but not twisting, of the DNA and further point to the propagation of a bent region (loop or bulge) from one end of the nucleosome to the other.

PMID:
15677336
PMCID:
PMC546017
DOI:
10.1073/pnas.0409413102
[Indexed for MEDLINE]
Free PMC Article

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