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Exp Hematol. 2005 Feb;33(2):182-8.

Hematopoietic perturbation in zebrafish expressing a tel-jak2a fusion.

Author information

1
Centre for Cellular & Molecular Biology, School of Biological & Chemical Sciences, Deakin University, Burwood, Victoria, Australia.

Abstract

OBJECTIVE:

Various TEL-JAK2 fusions have been identified in patients with lymphoblastic and myeloid leukemias that result in constitutive activation of the JAK2 kinase domain. Such fusions can mediate factor-independent growth of hematopoietic cell lines and induction of malignancy in mouse models.

MATERIALS AND METHODS:

To assess whether zebrafish could be utilized as a suitable model for the study of myeloid oncogenesis, we generated a zebrafish tel-jak2a fusion oncoprotein based on that seen in a case of chronic myeloid leukemia. This was transiently expressed in zebrafish embryos under the control of the spi1 promoter, which is strongly active in myeloid precursors.

RESULTS:

Visual, histological, and molecular analysis revealed disruption of normal embryonic hematopoiesis, including perturbation of the myeloid and erythroid lineages.

CONCLUSION:

These results indicate that the zebrafish tel-jak2a oncoprotein is functional, and suggest that this organism will be useful for the experimental study of myeloid malignancy.

PMID:
15676212
DOI:
10.1016/j.exphem.2004.10.019
[Indexed for MEDLINE]

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