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FEMS Microbiol Lett. 2005 Feb 1;243(1):259-63.

Sensitive and rapid detection of Shigella and enteroinvasive Escherichia coli by a loop-mediated isothermal amplification method.

Author information

1
Division of Bacterial Pathogenesis, Department of Microbiology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan. k018763@med.u-ryukyu.ac.jp

Abstract

Here we report a loop-mediated isothermal amplification (LAMP) method for detecting Shigella and enteroinvasive Escherichia coli. The target for this LAMP method is the ipaH gene which is carried by both of the pathogens. The LAMP method efficiently detected the gene within 2 h at a minimal amount of bacteria (8 CFU) per reaction.

PMID:
15668027
DOI:
10.1016/j.femsle.2004.12.014
[Indexed for MEDLINE]
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