Format

Send to

Choose Destination
See comment in PubMed Commons below
FEMS Microbiol Lett. 2005 Feb 1;243(1):51-8.

The riboflavin kinase encoding gene ribR of Bacillus subtilis is a part of a 10 kb operon, which is negatively regulated by the yrzC gene product.

Author information

1
Molecular and Radiation Biophysics Division, St. Petersburg Nuclear Physics Institute of the Russian Academy of Sciences, Gatchina, Leningrad district 188350, Russia. solovieva@em.uni-frankfurt.de

Abstract

The riboflavin kinase encoding gene ribR is situated within a 12 genes locus ytmI-ytnM of the Bacillus subtilis chromosome. Here we demonstrate that ribR is transcribed as part of a 10 kb ytmI-ytnM operon. The riboflavin overproduction phenotype of B. subtilis ribC mutant strains, which is a result of the strongly reduced flavokinase activity of the riboflavin kinase/FAD synthetase RibC, was suppressed by ribR expression. Analysis of mutations with an upregulated ribR gene revealed 2 different groups of mutants. One class of mutants contained base substitutions in an 8 nucleotide sequence of the promoter region of the ytmI-ytnM operon. A second class of mutants had single point mutations within the yrzC gene or in the RBS of this gene. Dot-blot analysis of ytmI-ytnM transcription and the results of in trans complementation experiments for the yrzC mutants confirmed a role of the yrzC gene product as a negative regulator for the ytmI-ytnM operon.

PMID:
15668000
DOI:
10.1016/j.femsle.2004.11.038
[Indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Silverchair Information Systems Icon for Wiley
    Loading ...
    Support Center