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J Bacteriol. 2005 Feb;187(3):1192-5.

Characterization of phospholipase activity of the Pseudomonas aeruginosa type III cytotoxin, ExoU.

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1
Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Rd., Milwaukee, WI 53226, USA.

Abstract

Recombinant ExoU (rExoU) and yeast extract were used to optimize an in vitro phospholipase assay as a basis for identifying the mechanism for enzyme activation and substrate specificity. Our results support a model in which a eukaryotic protein cofactor or complex facilitates the interaction of rExoU with phospholipid substrates.

PMID:
15659695
PMCID:
PMC545705
DOI:
10.1128/JB.187.3.1192-1195.2005
[Indexed for MEDLINE]
Free PMC Article
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