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J Biochem Biophys Methods. 2005 Jan 31;62(1):69-79. Epub 2004 Dec 19.

Analytical and semipreparative resolution of enatiomers of albendazole sulfoxide by HPLC on amylose tris (3,5-dimethylphenylcarbamate) chiral stationary phases.

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  • 1Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China. zzdlz@yahoo.com.cn

Abstract

Broad spectrum anthelmintic agent-albendazole sulfoxide (ABZSO) have been separated and semiprepared on amylose tris (3,5-dimethylphenylcarbamate) chiral stationary phases by HPLC using mobile phases contained with n-hexane and different alcohols. For analytical separation the influence of the nature and content of alcoholic modifiers on separation were systemically studied. Then, the analytical methods were scaled up to semipreparative loading to obtain small quantities (about 1 g) of both ABZSO enantiomers. Especially, different loading amounts were investigated for their effect on various parameters of semipreparative HPLC. In addition, optical rotation and circular dichroism (CD) of both ABZSO enantiomers collected were determined and single enantiomers were found stable in configuration for 1 year.

PMID:
15656945
DOI:
10.1016/j.jbbm.2004.09.006
[PubMed - indexed for MEDLINE]
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