Identification of a novel member of yeast mitochondrial Hsp70-associated motor and chaperone proteins that facilitates protein translocation across the inner membrane

Hayashi Yamamoto; Takaki Momose; Yoh-Ichi Yatsukawa; Chié Ohshima; Daigo Ishikawa; Takehiro Sato; Yasushi Tamura; Yukimasa Ohwa; Toshiya Endo

doi:10.1016/j.febslet.2004.12.018

Identification of a novel member of yeast mitochondrial Hsp70-associated motor and chaperone proteins that facilitates protein translocation across the inner membrane

FEBS Lett. 2005 Jan 17;579(2):507-11. doi: 10.1016/j.febslet.2004.12.018.

Authors

Hayashi Yamamoto¹, Takaki Momose, Yoh-Ichi Yatsukawa, Chié Ohshima, Daigo Ishikawa, Takehiro Sato, Yasushi Tamura, Yukimasa Ohwa, Toshiya Endo

Affiliation

¹ Department of Chemistry, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.

PMID: 15642367
DOI: 10.1016/j.febslet.2004.12.018

Abstract

Here, we report the identification of yeast 15-kD Tim15/Zim17, a new member of mitochondrial Hsp70 (mtHsp70)-associated motor and chaperone (MMC) proteins. The 15-kD MMC protein is a peripheral inner membrane protein with a zinc-finger motif. Depletion of the 15-kD protein led to impaired import of presequence-containing proteins into the matrix in vivo and in vitro. Overexpression of the 15-kD protein rescued the functional defects of mtHsp70 in ssc1-3 cells, and a fusion protein containing the 15-kD protein physically interacts with purified mtHsp70. Tim15/Zim17 therefore cooperates with mtHsp70 to facilitate import of presequence-containing proteins into the matrix.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
HSP70 Heat-Shock Proteins / metabolism*
Intracellular Membranes / metabolism
Mitochondria / metabolism*
Mitochondrial Proteins / genetics
Mitochondrial Proteins / metabolism*
Mitochondrial Proteins / physiology
Molecular Chaperones / metabolism*
Molecular Sequence Data
Mutation / genetics
Protein Transport
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / metabolism*
Saccharomyces cerevisiae Proteins / physiology

Substances

HSP70 Heat-Shock Proteins
Mitochondrial Proteins
Molecular Chaperones
Saccharomyces cerevisiae Proteins
Zim17 protein, S cerevisiae