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Appl Environ Microbiol. 2005 Jan;71(1):39-45.

Bacillus subtilis alpha-phosphoglucomutase is required for normal cell morphology and biofilm formation.

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1
Département de Microbiologie Fondamentale, Batiment de Biologie, Faculté de Biologie et de Médecine, Université de Lausanne, CH-1015 Lausanne, Switzerland. vladimir.lazarevic@imf.unil.ch

Abstract

Mutations designated gtaC and gtaE that affect alpha-phosphoglucomutase activity required for interconversion of glucose 6-phosphate and alpha-glucose 1-phosphate were mapped to the Bacillus subtilis pgcA (yhxB) gene. Backcrossing of the two mutations into the 168 reference strain was accompanied by impaired alpha-phosphoglucomutase activity in the soluble cell extract fraction, altered colony and cell morphology, and resistance to phages phi29 and rho11. Altered cell morphology, reversible by additional magnesium ions, may be correlated with a deficiency in the membrane glycolipid. The deficiency in biofilm formation in gtaC and gtaE mutants may be attributed to an inability to synthesize UDP-glucose, an important intermediate in a number of cell envelope biosynthetic processes.

PMID:
15640167
PMCID:
PMC544238
DOI:
10.1128/AEM.71.1.39-45.2005
[Indexed for MEDLINE]
Free PMC Article
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