Format

Send to

Choose Destination
J Zhejiang Univ Sci B. 2005 Feb;6(2):132-6.

Representative appressorium stage cDNA library of Magnaporthe grisea.

Author information

1
School of Life Sciences, Zhejiang University, Hangzhou 310029, China.

Abstract

A mature appressorium cDNA library of rice blast fungus, Magnaporthe grisea, was constructed in a lambdaTriplEx2 vector by SMART cDNA library containing 2.37x10(6) independent clones about 100% of which harbor foreign cDNA inserts with average size of 660 bp. Of 9 randomly selected clones, 2 expressed sequence tags (ESTs) sequences did not have homologous EST sequences of M. grisea in GenBank. The appressorium cDNA library is suitable for gene expression analysis and function analysis of the late stages of appressorium formation and the early stages of penetration of M. grisea.

PMID:
15633249
PMCID:
PMC1389628
DOI:
10.1631/jzus.2005.B0132
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Zhejiang University Press Icon for PubMed Central
Loading ...
Support Center