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J Hepatol. 2005 Jan;42(1):61-7.

Comparable functions of plasmacytoid and monocyte-derived dendritic cells in chronic hepatitis C patients and healthy donors.

Author information

1
Immunology and Virology Department, Chiron Vaccines Research Center, via Fiorentina 1, 53100 Siena, Italy.

Abstract

BACKGROUND/AIMS:

Dendritic cells (DCs) play a key role in immune responses through antigen presentation and cytokine secretion. Hepatitis C virus (HCV) is able to escape elimination by the immune system and often establishes a chronic infection. To investigate whether DC dysfunction is involved in this process, we have studied monoycte-derived DCs (Mo-DCs) and plasmacytoid DCs (pDCs), which produce large amounts of IFN-alpha, from chronic HCV patients and healthy donors.

METHODS:

We have assessed TNF-alpha and IFN-alpha production by pDCs using intracellular staining after total PBMCs stimulation with unmethylated CG dinucleotides (CpGs). The induction of allogeneic T cell proliferation by immature Mo-DCs was measured using the MLR assay. The up-regulation of maturation markers and the production of TNF-alpha in response to LPS were analyzed using flow cytometry and ELISA, respectively.

RESULTS:

We have detected comparable frequencies of pDCs producing TNF-alpha and IFN-alpha in both chronic HCV patients and healthy donors and we have found that immature Mo-DCs from both patients and donors similarly induce allogeneic T cell proliferation and mature and secrete TNF-alpha in response to LPS.

CONCLUSIONS:

Our results demonstrate that both pDC and Mo-DCs are not impaired in HCV infected patients.

PMID:
15629508
DOI:
10.1016/j.jhep.2004.09.014
[Indexed for MEDLINE]
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