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Biochim Biophys Acta. 1992 Apr 6;1130(3):343-4.

Cloning of two adult hamster globin cDNAs (alpha and beta major).

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St. Luke's-Roosevelt Hospital Center, Hematology-Oncology Division, New York, NY 10025.


A cDNA library was prepared from poly(A) mRNA extracted from adult anemic hamster spleen erythroid cells. cDNA clones containing inserts coding for adult alpha and beta major globin chains were isolated. Their identity was confirmed by (a) translation of hybrid selected mRNA and (b) nucleotide sequence analysis of the inserts and comparison to the adult globin cDNAs of mouse, rabbit and human. Availability of sequences for embryonic (Li et al. (1992) Biochim. Biophys. Acta 1130, 218-220) and adult globin cDNAs (this report) will aid in investigations of the molecular mechanisms involved in the globin ontogeny of hamsters.

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