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Biochem Biophys Res Commun. 1976 Dec 20;73(4):985-92.

Particulate DNA polymerase from the cytoplasm of Xenopus laevis oocytes.

Author information

1
Laboratory of Cell Biology, C. N. R., Via Romagnosi 18A, 00196 Rome, Italy.

Abstract

A DNA polymerase has been partially purified and characterized from Xenopus laevis stage 6 oocytes. The enzyme is present only in the cytoplasm and has been shown to be able to copy Poly(A) x oligo(dT), to be sensitive to N-ethylmaleimide, and to sediment faster than 4 S in high salt glycerol gradient. The enzyme can be extracted from particulate material which has a density in sucrose gradient ranging from 1.200 to 1.225 g/cc. This particulate material is identified by its ability to use Poly(A) x oligo(dT) as template in an exogenous DNA polymerase reaction and by its endogenous DNA synthesizing capacity.

PMID:
15625871
DOI:
10.1016/0006-291x(76)90219-9
[Indexed for MEDLINE]

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