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Biotechnol Lett. 2004 Nov;26(22):1731-7.

Real Time RT-PCR and flow cytometry to investigate wheat kernel hardness: role of puroindoline genes and proteins.

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  • 1School of Biotechnological Sciences, University of Naples Federico II, Via Universit√† 133, Portici, Naples, 80055, Italy.

Abstract

Developing seeds from Triticum aestivum (wheat) cultivars were collected after flowering and analysed for puroindoline a and b gene expression by Real Time RT-PCR. Mature seeds were investigated for the presence and the amount of starch-associated puroindoline a and b proteins by flow cytometry. Puroindoline a gene and protein were found to have a predominant role in controlling wheat kernel hardness.

PMID:
15604827
DOI:
10.1007/s10529-004-3745-3
[PubMed - indexed for MEDLINE]
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