Use of a monoclonal antibody against Babesia bovis merozoite surface antigen-2c for the development of a competitive ELISA test

Ann N Y Acad Sci. 2004 Oct:1026:165-70. doi: 10.1196/annals.1307.025.

Abstract

Bovine babesiosis caused by Babesia bovis is a disease that hampers the production of beef and dairy cattle in tropical and subtropical regions of the world. New diagnostic methods based on recombinant antigens constitute valuable biotechnological tools for the strategic control of this disease. We have developed a competitive enzyme-linked immunosorbent assay that includes a recombinant form of the merozoite surface antigen-2c and a novel monoclonal antibody against it. Preliminary results showed that this test is able to identify specific antibodies against B. bovis from experimentally and naturally infected cattle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Protozoan / analysis*
  • Antigens, Protozoan / immunology*
  • Babesia bovis / immunology*
  • Babesia bovis / pathogenicity*
  • Babesiosis / diagnosis*
  • Babesiosis / immunology*
  • Cattle
  • Cattle Diseases / diagnosis*
  • Cattle Diseases / immunology*
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Enzyme-Linked Immunosorbent Assay / veterinary
  • Membrane Proteins / analysis*
  • Membrane Proteins / immunology*
  • Protozoan Proteins / analysis*
  • Protozoan Proteins / immunology*

Substances

  • Antibodies, Monoclonal
  • Antigens, Protozoan
  • MSA-2 protein, Babesia bovis
  • Membrane Proteins
  • Protozoan Proteins