Format

Send to

Choose Destination
J Bacteriol. 2005 Jan;187(1):396-9.

Assimilation of nitrogen from nitrite and trinitrotoluene in Pseudomonas putida JLR11.

Author information

1
Consejo Superior de Investigaciones Cientificas, Estación Experimental del Zaidin, Department of Biochemistry and Molecular and Cellular Biology of Plants, Granada, Spain.

Abstract

Pseudomonas putida JLR11 releases nitrogen from the 2,4,6-trinitrotoluene (TNT) ring as nitrite or ammonium. These processes can occur simultaneously, as shown by the observation that a nasB mutant impaired in the reduction of nitrite to ammonium grew at a slower rate than the parental strain. Nitrogen from TNT is assimilated via the glutamine syntethase-glutamate synthase (GS-GOGAT) pathway, as evidenced by the inability of GOGAT mutants to use TNT. This pathway is also used to assimilate ammonium from reduced nitrate and nitrite. Three mutants that had insertions in ntrC, nasT, and cnmA, which encode regulatory proteins, failed to grow on nitrite but grew on TNT, although slower than the wild type.

PMID:
15601726
PMCID:
PMC538816
DOI:
10.1128/JB.187.1.396-399.2005
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center