Format

Send to

Choose Destination
FEMS Microbiol Lett. 2004 Dec 15;241(2):249-56.

Cytochrome c maturation proteins are critical for in vivo growth of Legionella pneumophila.

Author information

1
Department of Microbiology and Immunology, Northwestern University Medical School, 320 East Superior St., Chicago, IL 60611, USA.

Abstract

Legionella pneumophila, an intracellular parasite of macrophages and protozoa, requires iron for extra- and intracellular growth. In a new screen of a mutant library of L. pneumophila for strains defective for growth on agar media lacking supplemental iron, seven mutants were obtained. All of the mutants had a disruption in the cytochrome c maturation (ccm) locus; two had insertions in ccmB, two in ccmC, and three in ccmF. The ccm mutants were unable to multiply within macrophage-like cells (i.e., U937 and THP-1 cells) and Hartmannella vermiformis amoebae. A competition assay in A/J mice revealed that ccm mutants are severely defective for growth within the lung. Taken together, these data confirm that ccm and cytochrome c maturation proteins are required for L. pneumophila growth in low iron, intracellular infection, and virulence.

PMID:
15598540
DOI:
10.1016/j.femsle.2004.10.028
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for Wiley
Loading ...
Support Center