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Eur J Obstet Gynecol Reprod Biol. 2005 Jan 10;118(1):66-70.

Bypassing the effect of zona pellucida changes on embryo formation following cryopreservation of bovine oocytes.

Author information

  • 1School of Human Development, University of Nottingham, Queen's Medical Centre, Nottingham NG72UH, UK. andreas@mavrides.com

Abstract

OBJECTIVE:

To evaluate the effects of zona pellucida changes following the cryopreservation of bovine oocytes using two different techniques.

DESIGN:

Prospective randomised study.

SETTING:

University research facility and IVF research laboratory, NURTURE, University of Nottingham, UK.

INTERVENTION:

508 bovine oocytes were collected from abattoir-derived ovaries and cryopreserved by slow freezing using standard straws and a new vitrification method using our self-constructed cryoloops. After thawing, the oocytes were inseminated by ICSI and standard IVF.

MAIN OUTCOME MEASURES:

Oocyte degeneration and oocyte cleavage.

RESULTS:

Cleavage rates after IVF in fresh oocytes was higher (49.5%) than the cryopreservation groups (slow freezing: 15.4% and vitrification: 25.8%), whereas after ICSI, the rates were similar (fresh: 17.3%, slow freezing: 9.4%, vitrification: 16.0%).

CONCLUSIONS:

Zona pellucida changes after oocyte cryopreservation significantly reduce the embryo formation rates after IVF. ICSI bypasses this complication and in selected species it could be an appropriate method of insemination.

PMID:
15596275
DOI:
10.1016/j.ejogrb.2004.06.025
[PubMed - indexed for MEDLINE]
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