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Methods Mol Biol. 2005;296:355-69.

Polo-like kinase-1: activity measurement and RNAi-mediated knockdown.

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1
Division of Molecular Biology, Netherlands Cancer Institute, Amsterdam, The Netherlands.

Abstract

Polo-like kinase-1 (Plk-1) is an important cell cycle regulatory kinase that has been implicated in a multitude of cell cycle events. In this chapter we review those multiple functions of Plk-1 and describe the methods routinely used in our laboratory to purify Plk-1 from cellular lysates and measure Plk-1 kinase activity in vitro. In addition, we describe a method to analyze cell cycle progression after depletion of Plk-1 by RNA-interference in tissue culture cells.

PMID:
15576944
[Indexed for MEDLINE]

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