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Methods Mol Biol. 2005;294:321-34.

Application of microscope-based FRET to study molecular interactions in focal adhesions of live cells.

Author information

1
Department of Moelcular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel.

Abstract

This chapter describes the use of microscope-based fluorescence resonance energy transfer to follow dynamic interaction of molecules localized at focal adhesions. We first outline the significance of studying dynamic interactions in focal adhesions of living cells, and second provide an overview of the method itself. This is followed by a protocol for fluorescence resonance energy transfer measurements in live cells.

PMID:
15576921
DOI:
10.1385/1-59259-860-9:321
[Indexed for MEDLINE]

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