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Appl Environ Microbiol. 2004 Dec;70(12):7578-80.

Association of Escherichia coli O157:H7 with houseflies on a cattle farm.

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1
Department of Entomology, Kansas State University, Manhattan, KS 66506, USA.

Abstract

The ecology of Escherichia coli O157:H7 is not well understood. The aims of this study were to determine the prevalence of and characterize E. coli O157:H7 associated with houseflies (HF). Musca domestica L. HF (n = 3,440) were collected from two sites on a cattle farm over a 4-month period and processed individually for E. coli O157:H7 isolation and quantification. The prevalence of E. coli O157:H7 was 2.9 and 1.4% in HF collected from feed bunks and a cattle feed storage shed, respectively. E. coli O157:H7 counts ranged from 3.0 x 10(1) to 1.5 x 10(5) CFU among the positive HF. PCR analysis of the E. coli O157:H7 isolates revealed that 90.4, 99.2, 99.2, and 100% of them (n = 125) possessed the stx1, stx2, eaeA, and fliC genes, respectively. Large populations of HF on cattle farms may play a role in the dissemination of E. coli O157:H7 among animals and to the surrounding environment.

PMID:
15574966
PMCID:
PMC535191
DOI:
10.1128/AEM.70.12.7578-7580.2004
[Indexed for MEDLINE]
Free PMC Article
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