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J Biochem Biophys Methods. 2004 Nov 30;61(3):265-70.

Exploring permeability of Escherichia coli competence using quantum dots as fluorescent probes.

Author information

1
College of Life Sciences, Wuhan University, Wuhan 430072, PR China.

Abstract

Though people had recognized the pivotal function of CaCl(2) during DNA transformation into Escherichia coli, the mechanism of divalent Ca(2+) cation inducing E. coli competence development is still unknowable. Quantum dots (QDs), as a new fluorescent probe, being applied in biology research, had aroused great interest. We explored the penetrability of E. coli competent cells membrane using QDs and proved directly that competent cells were more permeable than that of noncompetent. The results are significant on understanding the problems of the microbiological genetics.

PMID:
15571775
DOI:
10.1016/j.jbbm.2003.10.005
[Indexed for MEDLINE]

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