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BMC Microbiol. 2004 Nov 30;4:45.

DNA-free RNA preparations from mycobacteria.

Author information

1
Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr, 5, D-91058 Erlangen, Germany. jstephan@biologie.uni-erlangen.de <jstephan@biologie.uni-erlangen.de>

Abstract

BACKGROUND:

To understand mycobacterial pathogenesis analysis of gene expression by quantification of RNA levels becomes increasingly important. However, current preparation methods yield mycobacterial RNA that is contaminated with chromosomal DNA.

RESULTS:

After sonication of RNA samples from Mycobacterium smegmatis genomic DNA is efficiently removed by DNaseI in contrast to untreated samples.

CONCLUSIONS:

This procedure eliminates one of the most prevalent error sources in quantification of RNA levels in mycobacteria.

PMID:
15571628
PMCID:
PMC539233
DOI:
10.1186/1471-2180-4-45
[Indexed for MEDLINE]
Free PMC Article
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