Format

Send to

Choose Destination
Hepatobiliary Pancreat Dis Int. 2004 Nov;3(4):564-70.

Abnormal expression of hepatoma-derived gamma-glutamyltransferase subtyping and its early alteration for carcinogenesis of hepatocytes.

Author information

1
Research Center of Clinical Molecular Biology, Affiliated Hospital of Nantong University, Nantong 226001, China. dfyrcmb@yahoo.com

Abstract

BACKGROUND:

Although the hepatoma-specific band of gamma-glutamyltransferase (GGT) is a highly sensitive marker in diagnosis of hepatocellular carcinoma, the kinetic expression and the early alterations of GGT in the development of hepatoma remain unclear. In this study, we investigated the expression and the alterations of GGT multiple molecular forms in hepatotumorigenesis.

METHODS:

The expression of GGT in a chemically induced hepatocarcinogenesis model was examined by giving 0.05% of 2-fluoenylacetamide in diet for 12 weeks. The expression levels of total RNA and GGT, and the changes of liver pathology, GGT multiple molecular forms and sugar-chain heterogeneity were investigated at the different stages of rat hepatoma development.

RESULTS:

Pathological examination and biochemical analysis found that liver GGT was over-expressed and secreted into blood during canceration. Serum total GGT and liver GGT specific activities (IU/g) including soluble and membrane-combined GGT were significantly higher (P<0.05) in experimental groups than those in control group, respectively. A highly positive correlation was found between total GGT activities and total RNA levels (r=0.90, P<0.05) of the liver. Both were higher six weeks later than before. Con A-non-reactive-GGT was increased consistantly during the development of rat hepatoma. GGT multiple molecular forms in the liver and sera of experimental rats showed that fetal liver-type GGT bands were associated with the development of hepatoma.

CONCLUSIONS:

Fetal liver-type GGT in sera and the liver of rats is closely related to hepatotumorigenesis. It can be used as a sensitive enzymatic marker for the early diagnosis of liver cancer.

PMID:
15567746
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for First Affiliated Hospital, Zhejiang University School of Medicine
Loading ...
Support Center