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Clin Diagn Virol. 1993 Aug;1(3):179-85.

Antiviral susceptibility testing of cytomegalovirus: criteria for detecting resistance to antivirals.

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Mount Zion Medical Center of the University of California, San Francisco, San Francisco, CA 94120-7921, USA.


Testing cytomegaloviruses for antiviral susceptibility is increasing especially since the reports of 'resistance' to ganciclovir and foscarnet (Erice et al., 1989; Knox et al., 1991). There is however no standardized method for susceptibility testing nor are there criteria for designating an isolate as sensitive or resistant. In a previous paper we utilized a plaque reduction assay and suggested that a resistant strain be defined as one requiring > 12 microM ganciclovir for inhibition of 50% of viral plaques. (Drew et al., 1991) This concentration was chosen because it was at least four-fold greater than the mean concentration required to inhibit pretherapy isolates. In this paper we present the results of testing a large number of isolates prior to and during therapy with either ganciclovir or foscarnet. By analyzing the results of these assays we propose revised criteria for susceptibility of cytomegalovirus </= 5 microM for ganciclovir and <400 microM for foscarnet.

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