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J Biomol NMR. 2004 Oct;30(2):175-9.

Optimization of 13C direct detection NMR methods.

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University of California San Francisco, San Francisco, California 94143, USA.


(13)C-detected experiments are still limited by their inherently lower sensitivity, as compared to the equivalent (1)H-detected experiments. Improving the sensitivity of (13)C detection methods remains a significant area of NMR research that may provide better means for studying large macromolecular systems by NMR. In this communication, we show that (13)C-detected experiments are less sensitive to the salt concentration of the sample solution than (1)H-detected experiments. In addition, acquisition can be started with anti-phase coherence, resulting in higher sensitivity due to the elimination of the final INEPT transfer step.

[Indexed for MEDLINE]

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