Send to

Choose Destination
FEBS Lett. 2004 Nov 19;577(3):376-80.

Development of a tightly regulated U6 promoter for shRNA expression.

Author information

Cancer Research, Global Pharmaceutical Research and Development, AP10/L01, Abbott Laboratories, 100 Abbott Park Road, Abbott Park, IL 60064, USA.


Short hairpin RNAs (shRNAs) have been used to achieve stable target knockdown in a variety of biological systems. Here, we report the development of a tightly regulated tetracycline-responsive human U6 promoter for shRNA expression. By engineering two copies of the tet operators flanking the TATA box of the human U6 promoter, we created a U6 promoter derivative (2O2) that exhibited much lower basal transcriptional activity compared with recently reported inducible pol III dependent promoters. As a consequence of its tighter regulation, the 2O2 system greatly improved the success rate in making inducible knockdown cell lines.

[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center