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[Study on the TCR Vbeta binding sites in the superantigen staphylococcal enterotoxin D].

[Article in Chinese]

Author information

1
Department of Epidemiology, Third Military Medical University, Chongqing 400038, China. yafeilye@yahoo.com.cn

Abstract

AIM:

To study TCR Vbeta binding sites of staphylococcal enterotoxin D(SED).

METHODS:

Six SED mutants were constructed by site-directed mutagenesis. The activity of promoting T cell proliferation by the mutants was detected by (3)H-TdR incorporation. For the mutants with decreased mitogenic activity, flow cytometry was used detect their MHC-II binding activity and TCR Vbeta specificity.

RESULTS:

Residue N23 played an important role in the interaction of SED with human TCR Vbeta5. Residue H26 was probably a SED binding site to human TCR Vbetas except for TCR Vbeta5, TCR Vbeta8 and TCR Vbeta12.1.

CONCLUSION:

Residue N23 is a key TCR Vbeta binding site of SED.

PMID:
15555455
[Indexed for MEDLINE]

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