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J Allergy Clin Immunol. 2004 Nov;114(5):1106-9.

Sputum and bronchial submucosal IL-13 expression in asthma and eosinophilic bronchitis.

Author information

1
Institute for Lung Health, University Hospitals of Leicester, Groby Road, Leicester LE3 9QP, UK.

Abstract

BACKGROUND:

Nonasthmatic eosinophilic bronchitis is a condition characterized by the presence of eosinophilic airway inflammation in the absence of airflow obstruction or airway hyperresponsiveness. In asthma, the T H 2-type cytokine IL-13 has been implicated in the development of airway inflammation and hyperresponsiveness. Whether the expression of IL-13 is different between these 2 conditions is unknown.

OBJECTIVE:

We sought to investigate whether IL-13 expression is increased in asthma compared with eosinophilic bronchitis.

METHODS:

Sputum samples from subjects with mild asthma (n = 30) and eosinophilic bronchitis (n = 15) and normal controls (n = 16) were dialyzed, and IL-13 concentration was measured by ELISA. In a subgroup of these patients, IL-13 protein expression in bronchial biopsies was assessed by immunohistochemistry.

RESULTS:

The concentration of sputum IL-13 was higher in patients with mild asthma than in normal controls ( P = .03) and in patients with eosinophilic bronchitis ( P = .03). The median (interquartile range) number of IL-13 + cells/mm 2 submucosa was significantly higher in asthma 4 (8) than eosinophilic bronchitis 1.7 (1.9) and normal controls 0.5 (1.1; P = .004). Eighty-three percent of the cells expressing IL-13 in the submucosa were eosinophils, and 8% were mast cells. The median (interquartile range) proportion of eosinophils that expressed IL-13 was higher in the subjects with asthma, 16 (10)%, than those with eosinophilic bronchitis, 7 (3)% ( P = .02).

CONCLUSION:

The increased expression of IL-13 in asthma compared with eosinophilic bronchitis supports the concept that IL-13 may play a critical role in the pathophysiology of asthma.

PMID:
15536417
DOI:
10.1016/j.jaci.2004.08.032
[Indexed for MEDLINE]

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