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Vaccine. 2004 Dec 2;23(3):404-10.

Expression of a vaccine protein in a plant cell line using a geminivirus-based replicon system.

Author information

1
Cornell Research Foundation, Cornell University, 20 Thornwood Dr., Ithaca, NY 14850, USA. klh22@cornell.edu

Abstract

Edible vaccines have been generated from both transgenic plants as well as from plant viral vectors. Here, we have taken the best attributions of both systems and designed a minimalized version of the bean yellow dwarf geminivirus (BeYDV)-based replicon consisting of the cis-acting elements required for BeYDV replication as a means to express foreign genes in a plant cell line. Replication can be switched on at high levels upon expression of the BeYDV Rep protein, and gene expression enhanced enormously. Construction of an expression cassette encoding a synthetic vaccine gene and analysis of expression levels of a vaccine protein in a plant cell line system are described.

PMID:
15530687
DOI:
10.1016/j.vaccine.2004.04.038
[Indexed for MEDLINE]
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