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Appl Environ Microbiol. 2004 Nov;70(11):6884-6.

Evaluation of the LIVE/DEAD BacLight kit for detection of extremophilic archaea and visualization of microorganisms in environmental hypersaline samples.

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1
Cyanobacteria and Astrobiology Research Laboratory, University of New South Wales, Sydney, Australia.

Abstract

Extremophilic archaea were stained with the LIVE/DEAD BacLight kit under conditions of high ionic strength and over a pH range of 2.0 to 9.3. The reliability of the kit was tested with haloarchaea following permeabilization of the cells. Microorganisms in hypersaline environmental samples were detectable with the kit, which suggests its potential application to future extraterrestrial halites.

PMID:
15528557
PMCID:
PMC525124
DOI:
10.1128/AEM.70.11.6884-6886.2004
[Indexed for MEDLINE]
Free PMC Article
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