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Vaccine. 2004 Nov 15;23(1):48-57.

Comparative sequence analysis of Bordetella bronchiseptica pertactin gene (prn) repeat region variants in swine vaccines and field isolates.

Author information

1
Respiratory Diseases of Livestock Research Unit, USDA/Agricultural Research Service/National Animal Disease Center, P.O. Box 70, Ames, IA 50010, USA. kregiste@nadc.ars.usda.gov

Abstract

The product of the Bordetella bronchiseptica pertactin gene, prn, has been implicated as an adhesin and a protective immunogen in swine. Recent studies demonstrate prn sequence heterogeneity in swine isolates and vaccine strains within and surrounding the region 1 amino acid repeat GGXXP(n) and the region 2 amino acid repeat PQP(n). However, only a few isolates have been evaluated. Allelic variation between vaccine strains and field isolates may affect vaccine efficacy, since region 2 is known to encode an immunodominant protective epitope. In the present study, the DNA and predicted amino acid sequences of the pertactin repeat regions from a collection of 81 recent swine field isolates and 5 vaccine strains from the United States were determined. Two region 1 variants and four region 2 variants, one of which has not been previously reported, were identified, comprising four pertactin types. Four vaccines are derived from strains with a region 1 variant identical to that found in the majority of field isolates. However, only two vaccines possess the most commonly identified sequence in region 2, while two others contain a variant found in only one other swine isolate. Ribotype analysis demonstrated that although vaccines containing the novel region 2 variant fall within the same major cluster as other common swine ribotypes, they are less closely related. No relationship was observed between pertactin type and ribotype.

PMID:
15519707
DOI:
10.1016/j.vaccine.2004.07.020
[Indexed for MEDLINE]

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