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Clin Chem. 2005 Jan;51(1):125-31. Epub 2004 Oct 28.

Plasma F2-isoprostanes and coronary artery calcification: the CARDIA Study.

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Department of Laboratory Medicine and Pathology, School of Medicine, School of Public Health, University of Minnesota, Minneapolis, MN 55455, USA.



Oxidation of lipids in lipoproteins and cells may initiate and enhance the early development of cardiovascular disease.


We assayed F(2)-isoprostanes, oxidation products of arachidonic acid, by gas chromatography-mass spectrometry in a biracial cohort of 2850 young healthy adult men and women. Coronary artery calcification (CAC), a component of coronary artery atherosclerosis, was detectable in 10% of the cohort and appeared to be in its initial stages (Agatston scores <20 in 47% and <100 in 83% of CAC-positive participants). After adjusting for sex, clinical site, age, and race, the presence of any CAC was 24% more likely among those with high vs low concentrations of F(2)-isoprostanes [odds ratio (OR) = 1.24 per 92.2 pmol/L (32.7 ng/L; 1 SD of F(2)-isoprostanes); 95% confidence interval (CI), 1.09-1.41]. The OR was only slightly attenuated [1.18 per 92.2 pmol/L (32.7 ng/L); CI, 1.02-1.38] after further adjustment for body mass index, smoking, serum lipids, C-reactive protein, antioxidant supplementation use, diabetes, and blood pressure. As a continuous variable, the Agatston score increased by 6.9% per 92.2 pmol/L (32.7 ng/L) of F(2)-isoprostane concentration (P <0.01). Whereas CAC prevalence was lower in women than men, mean (SD), F(2)-isoprostanes were higher in women {190 (108.9) pmol/L [67.4 (38.6) ng/L]} than in men {140.4 (55.6) pmol/L [49.8 (19.7) ng/L]}. Nevertheless, F(2)-isoprostanes were associated with an increased risk of CAC in both sexes.


This association between increased concentrations of circulating F(2)-isoprostanes and CAC in young healthy adults supports the hypothesis that oxidative damage is involved in the early development of atherosclerosis.

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