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Klin Oczna. 2004;106(1-2 Suppl):155-7.

[The expression of Bcl-2, Bcl-xl, Bak and Bax proteins in axons of the optic nerve in closed-angle glaucoma].

[Article in Polish]

Author information

1
Kliniki Okulistyki Akademii Medycznej w Białymstoku.

Abstract

PURPOSE:

The aim of our study was the evaluation of Bcl-2, Bcl-xl, Bak and Bax immunoexpression in axons of the optic nerve with absolute glaucoma.

MATERIAL AND METHODS:

This study was conducted on 19 patients with absolute glaucoma, suffered from severe ophthalmalgia, treated of necessity by enucleation in 1991-2003 in our Department of Ophthalmology. The samples were immuno-stained with antibodies for Bcl-2, Bak, Bax and Bcl-xl protein. The reactions were performed in Labelled Streptavidin Avidin Biotin (LSAB) technique. The evaluation of immuno-staining was calculated as the percentage of cells with positive reaction to the total number of cells encountered in 10 representative fields, by light microscopy using a x 20 objective lens. Scores were based on the following scale: (++), over 50% of the field showing positive immuno-staining, (+) 10-50% with positive immunostaining.

RESULTS:

In our study proapoptotic Bak and Bax protein expression was stronger than antiapoptotic Bcl-2, Bcl-xl protein expression; Bak/Bcl-2 (p = 0.008), Bax/Bcl-2 (p = 0.0007), Bak/Bcl-xl (p = 0.0356), Bax/Bcl-xl (p = 0.0077).

CONCLUSIONS:

The results of our study demonstrate high proapoptotic trends in axons of the optic nerve in the stage of absolute glaucoma.

PMID:
15510481
[Indexed for MEDLINE]

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