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Am J Pathol. 2004 Nov;165(5):1799-807.

Quantitative gene expression profiling in formalin-fixed, paraffin-embedded tissues using universal bead arrays.

Author information

1
Genetic Analysis, Illumina, Inc., 9885 Towne Center Drive, San Diego, CA 92121, USA.

Abstract

We recently developed a sensitive and flexible gene expression profiling system that is not dependent on an intact poly-A tail and showed that it could be used to analyze degraded RNA samples. We hypothesized that the DASL (cDNA-mediated annealing, selection, extension and ligation) assay might be suitable for the analysis of formalin-fixed, paraffin-embedded tissues, an important source of archival tissue material. We now show that, using the DASL assay system, highly reproducible tissue- and cancer-specific gene expression profiles can be obtained with as little as 50 ng of total RNA isolated from formalin-fixed tissues that had been stored from 1 to over 10 years. Further, tissue- and cancer-specific markers derived from previous genome-wide expression profiling studies of fresh-frozen samples were validated in the formalin-fixed samples. The DASL assay system should prove useful for high-throughput expression profiling of archived clinical samples.

PMID:
15509548
PMCID:
PMC1618650
DOI:
10.1016/S0002-9440(10)63435-9
[Indexed for MEDLINE]
Free PMC Article

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