Format

Send to

Choose Destination
See comment in PubMed Commons below
Vet Immunol Immunopathol. 2004 Dec 8;102(3):143-54.

Infectious cDNA clones of porcine reproductive and respiratory syndrome virus and their potential as vaccine vectors.

Author information

1
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ont., N1G 2W1, Canada. dyoo@uoguelph.ca

Abstract

Full-length infectious cDNA clones have recently become available for both European and North American genotypes of porcine reproductive and respiratory syndrome virus (PRRSV), and it is now possible to alter the PRRSV genome and create genetically defined mutant viruses. Among many possible applications of the PRRSV infectious cDNA clones, development of genetically modified vaccines is of particular interest. Using infectious clones, the PRRSV genome has been manipulated by changing individual amino acids, deleting coding regions, inserting foreign sequences, and generating arterivirus chimeras. The limited available data suggest that all structural proteins of PRRSV are essential for replication of the virus, and that PRRSV infectivity is relatively intolerant of subtle changes within the structural proteins. The major tasks in PRRSV research are to identify virulence factors and pathogenic mechanisms, and to understand the structure-function relationships of individual viral proteins. Utilizing these infectious clones as tools, a new generation of safe and efficacious PRRS vaccines may be constructed.

PMID:
15507301
DOI:
10.1016/j.vetimm.2004.09.019
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center