Format

Send to

Choose Destination
Allergy. 2004 Dec;59(12):1277-84.

Bet v 1 homologues in strawberry identified as IgE-binding proteins and presumptive allergens.

Author information

1
SIK--The Swedish Institute for Food and Biotechnology, Gothenburg, Sweden.

Abstract

BACKGROUND:

No strawberry allergen has so far been identified and characterized.

METHODS:

Serum samples were collected from patients with a suggestive case history of adverse reactions to strawberry and other fruits. Extracts from fresh and frozen strawberries were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting and mass spectrometry. Patient blood samples were analysed for inhibition of IgE binding and basophil degranulation.

RESULTS:

Several IgE-binding proteins could be detected. In more than half of the patient sera, a 20/18-kDa doublet band was observed in Western blotting. These two bands were excised and analysed by mass spectrometry showing the presence of proteins belonging to the Bet v 1 family of allergens. Inhibition of the IgE binding to the 20/18-kDa doublet was obtained by addition of two recombinantly expressed allergens belonging to the Bet v 1 family (Bet v 1 and Mal d 1) and strawberry protein extract. In a cell-based assay of patient blood samples, basophil degranulation could be induced by strawberry protein extract and by Bet v 1 and Mal d 1.

CONCLUSIONS:

We conclude that strawberry homologues to Bet v 1 may be allergens of importance for adverse reactions to strawberry.

[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center